Coding
GpD

Part:BBa_K3308043

Designed by: Jemy Varghese, Ripal Sheth, Victor So, Harrison Green, Melissa Marciesky   Group: iGEM19_Pittsburgh   (2019-10-05)


Extein sequence bacteriophage λ head protein D (gpD)

We decided that bacteriophage λ head protein D (gpD)would be a viable extein sequence, utlizing it's well characterize structure and previous usage as an extein in intein research.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 171
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 171
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 171
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 171
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Zettler, J., Schütz, V., & Mootz, H. D. (2009). The naturally splitNpuDnaE intein exhibits an extraordinarily high rate in the proteintrans-splicing reaction. FEBS Letters, 583(5), 909–914. doi: 10.1016/j.febslet.2009.02.003


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Categories
Parameters
None